Fig. 8

dECM@exo inhibits ICH-induced excessive inflammation through the TLR4/NF-κB signaling pathway. (A) Western blot of TLR4 and NF-κB/P65, p-P65. (B) Statistical analysis of TLR4. (C) Statistical analysis of TLR4 mRNA. (D-F) ELISA analysis of IL-1β, TNF-α and IL-10 levels in different groups (n = 3). (G) Representative immunofluorescence images and (I) statistical analysis of TLR4 expression in astrocytes (n = 3). (H) Representative images and (J) statistical analysis of P-65 nuclear translocation in ICH-induced astrocytes (n = 3). Scale bars: G and H 100 μm. Data were expressed as mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001