Table 5 Methodological comparison
Method | Advantages | Limitations | AR-Specific Recommendations |
|---|---|---|---|
Ultracentrifugation | High purity, low cost | Aggregation, protein contamination | Combine with SEC for nasal delivery |
Commercial Kits | Fast, user-friendly | Co-isolates contaminants | Avoid for miRNA profiling |
Size-Exclusion Chromatography | Preserves vesicle integrity | Low yield, equipment-dependent | Ideal for functional in vivo studies |