Fig. 2

UA promotes exosome secretion from renal tubular epithelial cells and activates fibroblasts. A Schematic diagram of the experimental process. Exosomes from NRK-52E cells without treatment (Ctrl-Exo) or treatment with UA (UA-Exo) were extracted and incubated with NRK-49 F cells. B TEM image of exosomes isolated from NRK-52E cells. Scale bar = 100 nm. C NTA of exosomes from NRK-52E cells. D, E Representative western blots (D) and quantitative data (E) of CD63, Hsp70, and TSG101 in Ctrl-Exo and UA-Exo. F Fluorescent staining images of NRK-52E cell–derived exosomes taken up by NRK-49 F cells. Scale bars = 50 μm. G, H Representative western blots (G) and quantitative data (H) of CD63, Hsp70, and TSG101 in NRK-49 F cells incubated with exosomes from UA- or siRNA Rab27a–treated NRK-52E cells (n = 3). *p < 0.05 versus Ctrl-Exo, #p < 0.05 versus UA-Exo. I, J Representative western blots (I) and quantitative data (J) of Col-III, α-SMA, FN, and E-cad in NRK-49 F cells incubated with exosomes from UA- or siRNA Rab27a–treated NRK-52E cells (n = 3). *p < 0.05 versus Ctrl-Exo, #p < 0.05 versus UA-Exo. K, L Representative immunofluorescence micrographs (K) and quantitative data (L) showing Col-III and FN expression (n = 3). Scale bars = 50 μm. *p < 0.05 versus Ctrl-Exo, #p < 0.05 versus UA-Exo