Fig. 4

RTEC-derived exosome miR-330-3p promotes fibroblast activation. A Gene expression profiling using RNA-seq shows differential gene clustering of exosomes from NRK-52E cells with or without UA treatment. B Volcano plot showing differentially expressed genes of the 2 groups as indicated. C-G Relative expression of the top 5 miRNAs among RNA-Seq expression upregulated miRNAs in exosomes from NRK-52E cells with or without UA treatment. H-L Relative expression of the top 5 miRNAs among RNA-seq expression upregulated miRNAs in urinary exosomes in healthy controls and patients with CKD. M miR-330-3p levels in renal intrinsic cell-derived exosomes. N miR-330-3p levels in NRK-52E-Exo, NRK-52E cells, and NRK-52E cells Exo-out. O Simple linear regression between miR-330-3p from urinary exosomes in patients with CKD and the eGFR in patients with CKD. P ROC analysis of the combination of urinary exosomal miR-330-3p for discriminating CKD, an area under the ROC curve of 0.7956