Fig. 5

CREBBP is a potential target of miR-330-3p. A FISH and immunofluorescence staining confirming the intracellular transfer of tubule-derived exosomal miR-330-3p and its co-localization with the receptor of CD63 in NRK-52E cells. miR-330-3p (green), CREBBP (red), Scale bars = 50 μm. B Experimental design. C, D Representative western blots (C) and quantitative data (D) of Col-III, FSP1, and PCNA in NRK-49 F cells after stimulation with NRK-52E–delivered exosomes. *p < 0.05 versus Sham, #p < 0.05 versus UA-Exo. ^△p < 0.05 versus UA-Exo + miR-330-3p mimics. E, F Representative immunofluorescence micrographs (E) and quantitative data (F) showing Col-III and FN expression in NRK-49 F cells after stimulation with NRK-52E–delivered exosomes. Scale bars = 50 μm. *p < 0.05 versus Sham, #p < 0.05 versus UA-Exo. ^△p < 0.05 versus UA-Exo + miR-330-3p mimics. G-I CREBBP is a potential target of miR-330-3p. (G) Predicted potential miR-330-3p binding site in 3′-UTR of CREBBP mRNA. (H) Luciferase activity in NRK-49 F cells transfected with NC or miR-330-3p mimics together with reporter vector–containing CREBBP-mut binding sequences. #p < 0.05 versus miR-NC. ns, no significant difference. (I) Binding of CREBBP to miR-330-3p in NRK-49 F cells was detected using RAP-qPCR. K, L Representative western blots (K) and quantitative data (L) of CREBBP in NRK-49 F cells after stimulation with NRK-52E–delivered exosomes. *p < 0.05 versus Sham, #p < 0.05 versus UA-Exo. ^△p < 0.05 versus UA-Exo + miR-330-3p mimics