Fig. 2

Quantitative evaluation of the effect of ISO on the cellular source of TNTs. (A) CellTracker Green CMFDA-labeled CMs (green) and MSCs were co-cultured for 24 h, followed by WGA555 (red). TNTs derived from CMs or MSCs, which were indicated by arrow heads and arrow tips, respectively, were checked under a 40 × lens. (B) The ratio of different cellular originations of TNTs was quantified by randomly observing 94 and 118 TNTs with or without ISO. N = 4, scale bar: 50 μm. Data are shown as the mean ± S.E.M. *P < 0.05 vs Ctrl