Fig. 3

The effect of ISO on the cytoskeleton in TNTs. 24 h-co-cultured cells were stimulated by ISO, followed labelled by WGA 488 (green) and TnTs (blue). (A) F-actin were labeled with TRITC Phalloidin (red), 175 TNTs were randomly observed under a 40 × lens. Arrows heads indicate TNTs containing F-actin, and yellow triangles indicate F-actin in TNTs. N = 4, Scale bar: 50 μm. (B) Representative transmission electron micrograph of microtubules in TNTs. Red triangles marked the microtubules in TNTs between the cells. Microtubule, M. scale bar: 2 μm and 200 nm. (C) Microtubules were stained by α-tubulin antibodies (red). TNTs with (+) or without (-) microtubules were indicated by arrows heads and arrow tips, respectively. White triangles indicate microtubules in TNTs. (D) Randomly observe 132 TNTs, and quantitative percentage of TNTs containing microtubules. N = 5, Scale bar: 75 μm. Data are shown as the mean ± S.E.M.** 0.001 < P < 0.05