Fig. 4

Compare the suppressing effects of MSCs on ISO induced myocardial hypertrophy under different culture conditions. (A, B) The images represent immunostaining with TnT (Green), and the average cell surface area were quantified by detecting CMs in 10 randomly selected fields in four separate experiments. Scale bar: 100 μm. (C, D) Sorting CellTracker Green CMFDA-labeled CMs (Green) in co-culture system by flow cytometry, and the mRNA level of hypertrophic genes BNP, and β-MHC in CMs were analyzed by qPCR. N = 4, Data are shown as the mean ± S.E.M. * P < 0.05, ** 0.001 < P < 0.05 and ***P < 0.001 vs Ctrl