Fig. 8

Effect of knocking down Cx43 on MSCs to alleviate myocardial hypertrophy function. (A) Detecting the knock down efficiency of Cx43 SiRNA#1 and #2 (Si#1 and Si#2) in MSCs by western blot (WB). N = 3. (B) MSCs, pretreated with SiRNA control or Cx43 SiRNA, co-cultured with CMs for 24 h. CMs were labeled by TnT (Green). N = 4, Scale bar: 100 μm.(C) Quantifying the average surface area of CMs in 10 randomly selected fields. N = 4, Data are shown as the mean ± S.E.M. * P < 0.05 vs Ctrl, ** 0.001 < P < 0.05