Fig. 3
From: SERCA-mediated endoplasmic reticulum stress facilitates hematopoietic stem cell mobilization
ER Stress triggers calcium signaling pathway to regulate CXCR4 expression. (A-B) Downregulation of CXCR4 surface expression in SERCA2/3 knockdown Jurkat cells (n = 3). (C) The reduced CXCR4 expression could not be restored by the SERCA agonist (CDN1163) in SERCA2/3 knockdown Jurkat cells (n = 3). (D) Flow cytometric analysis of intracellular calcium transients in BHQ-treated Jurkat cells (n = 3). (E) Western blot analysis of p-CaMKII and CaMKII protein levels following BHQ treatment. Full-length blots are presented in Supplementary Fig. 6. (F-G) Suppression of CXCR4 expression in Jurkat cells treated with BD (250 µM for 1.5 h) and KN93 (100 µM for 5 h), similar to the effect of BHQ (n = 3). (H-I) Suppression of CXCR4 expression in mouse LSKs and HSCs by CaMKII inhibitors (n = 3)