Fig. 2

Differentiation and identification of mesenchymal stem cells derived from hPSC-NCC (hPSC-NCC-MSC). A. Scheme illustration of two maintenance culture mediums of NCC. B. Cell morphology of hPSC-NCC at passage 6 in N2B27 and N2B27 + LC culture medium. Scale bar: 500 μm. C. NCC markers P75, HNK1, SOX10 and AP2α mRNA levels of hPSC-NCC at passage 3 and 6 were analyzed by qPCR. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 N2B27 vs. N2B27 + LC. D. Scheme illustration of hPSC-NCC-MSC differentiation. E. Cell morphology of H1-NCC and H1-NCC-MSC. Scale bar: 500 and 200 μm. F. Representative histograms for stem cell (CD29, CD44, CD73, CD90, CD105) and hematopoietic stem cell (CD34, CD45) surface markers of H1-NCC-MSC analyzed by flow cytometry. The green color indicates the isotype control group, while the red color represents the hPSC-NCC-MSC group. G. Trilineage differentiation of H1-NCC-MSC. Scale bar: 100 μm. H. Adipocyte and osteoblast related genes were analyzed by qPCR. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 H1-NCC-MSC induced vs. UC-MSC induced. I. Cell proliferative rates were analyzed by CCK8 assay