Fig. 3

Comparison of viability and anti-senescence capability between H1-NCC-MSC and UC-MSC at different passages. A. Cell proliferative rates were analyzed by CCK8 assay. ^**p < 0.01, ^***p < 0.001 H1-NCC-MSC Passage 4 (P4) vs. UC-MSC Passage 4 (P4); ^##p < 0.01, ^###p < 0.001 H1-NCC-MSC Passage 15 (P15) vs. UC-MSC Passage 15 (P15). B-E. Ki67 immunostaining and quantitative analysis of Ki67 positive cell proportion (n = 3). Scale bar: 100 µm. F. Cell morphology. Scale bar: 200 μm. G-H. Representative images of aging-related β-galactosidase staining and quantitative analysis of positive cells (n = 3). Scale bar: 200 μm. I-J. γ-H2AX, P21 and P16 protein levels were quantified by Western blotting (n = 3). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. Full-length blots are presented in Additional file 2: Figure S6