Fig. 5

H1-NCC-MSC and UC-MSC asserted pro-viability and anti-apoptosis effect on GC in vivo and in vitro.A. MSC tracking in ovaries by DID-fluorescence probe. Scale bar: 100 μm. B-C. Apoptotic ovarian GC were evaluated by TUNEL assay and quantitative analysis of TUNEL positive cell ratio was conducted (n = 3). Scale bar: 100 μm. D-E. Proliferation indicator PCNA was immunostained and quantitative analysis was conducted for ovarian PCNA positive cells (n = 3). Scale bar: 50 μm. F. KGN cell morphology. Scale bar: 200 μm. G-H. Apoptotic rates of KGN detected using flow cytometry and quantitative analysis was conducted (n = 3). I. Proliferation rates were analyzed by CCK8. ^*p < 0.05, ^***p < 0.001 4-HC + H1-MSC-CM vs. 4-HC group; ^&&&p < 0.001 NC vs. 4-HC group; ^###p < 0.001 4-HC + UC-MSC-CM vs. 4-HC group. J-M. Protein levels of apoptosis-related genes γ-H2AX, BAX, BCL-2, Caspase 3 (CAS3) and Cleaved caspase 3 (C-CAS3) were quantified by Western blotting (n = 3). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. POI or 4-HC group. Full-length blots are presented in Additional file 2: Figure S7-S8.