Fig. 5

hUC-MSC-CM inhibits keratinocyte-derived chemokine secretion. A–C HaCaT cells treated with TNF-α/IFN-γ (10 ng/mL) or left untreated for 48 h. A Volcano plot showing the differential mRNA expression profiles of HaCaT cells (n = 3 biological replicates). B KEGG analysis of signaling pathways upregulated in TNF-α/IFN-γ-treated HaCaT cells. C Changes in chemokine expression in HaCaT cells following TNF-α/IFN-γ treatment. D–F HaCaT cells were exposed to TNF-α/IFN-γ (T/I; 10 ng/mL) or hUC-MSC-CM (50%, v/v) for 48 h. D Chemokine array analysis of cell supernatants, with mean pixel density quantified using ImageJ. E ELISA images showing CCL5 and CXCL11 protein levels in the supernatants. F qRT-PCR analysis of CCL5 and CXCL11 mRNA expression in HaCaT cells