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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Puerarin relives inflammation, bone destruction and facilitates osteogenic differentiation in periodontitis by enhancing mitochondrial autophagy via activating mitochondrial Mitofusin 2

Fig. 3

Pue treatment restored mitochondrial autophagy in the PD cell model. (A-B) TMRE staining combined with flow cytometry analysis was utilized to research mitochondrial membrane potential changes in hPDLCs. n = 3. (C-D) MitoSox Red staining combined with flow cytometry analysis was executed to monitor mitochondrial ROS level in hPDLCs. n = 3. (E-F) The expression of autophagy-related proteins in hPDLCs and mitochondria was detected by Western blot (Full-length blots/gels are presented in Supplementary Fig. 3E-LC3, Fig. 3E-P62, Fig. 3E-GAPDH, Fig. 3F-LC3, Fig. 3F-P62, Fig. 3F-Parkin, Fig. 3F-COXIV). n = 3. (G-H) LC3 dots in hPDLCs exhibited by mRFP-eGFP-LC3 assay. (I-J) LC3 dots in hPDLCs presented by Mito Tracker staining. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001. The “ns” symbol represented no obvious difference

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Stem Cell Research & Therapy

ISSN: 1757-6512