Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Puerarin relives inflammation, bone destruction and facilitates osteogenic differentiation in periodontitis by enhancing mitochondrial autophagy via activating mitochondrial Mitofusin 2

Fig. 4

Pue promoted osteogenic differentiation but attenuated inflammation of the PD cell model through enhancing mitochondrial autophagy (A-D) Alizarin red staining along with ALP staining was adopted to detect the osteogenic differentiation ability of hPDLCs. n = 3. (E-G) ELISA was carried out to test pro-inflammatory factors in hPDLCs. n = 3. (H) Osteogenesis-related genes in hPDLCs were examined by qRT-PCR. n = 3. (I-J) TMRE staining combined with flow cytometry analysis was employed to monitor mitochondrial membrane potential changes in hPDLCs. n = 3. (K-L) Mitochondrial ROS level in hPDLCs was appraised by MitoSox Red staining combined with flow cytometry analysis. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512