Fig. 5

Pue treatment maintained mitochondrial kinetic homeostasis in the PD cell model. (A-E) Mitochondrial dynamics-related factors in hPDLCs were scrutinized by qRT-PCR. n = 3. (F-G) Mfn2, Drp1, p-DRP1, OPA1, Mfn1 and Fis1 proteins in cytoplasm and mitochondria of hPDLCs were assessed by Western blot, respectively (Full-length blots/gels are presented in Supplementary Fig. 5F-Drp1, Fig. 5F-Mfn2, Fig. 5F-GAPDH, Fig. 5G-Drp1, Fig. 5G-Mfn2, Fig. 5G-COXIV). n = 3. (H-I) Mito Tracker staining was employed to research mitochondria division in hPDLCs. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001. The “ns” symbol represented no obvious difference