Table 1 m6A methylation modification regulates MSC osteogenic differentiation by targeting mRNAs
pathways | Regulators | Effects on osteogenesis | Mechanism | Species and cells | Ref. |
|---|---|---|---|---|---|
Wnt/β-catenin | METTL3↑ | promotion | P-Gsk-3β、β-catenin、 Lef1↑/Runx2↑ | Rat BMSCs | [79] |
METTL14↑ | promotion | PTPN6↑/ phosphorylation level of GSK-3β↑/β-catenin↑ | Human BMSCs | [80] | |
FTO↑、YTHDF2 | promotion | AGEs↑/FTO↑/SOST↓/ Wnt/β-catenin↑ | Mouse BMSCs | [81] | |
YTHDF1↑ | promotion | β-catenin↑/COL1 、 RUNX2↑ | Human BMSCs | [82] | |
BMP/Smad | METTL3↓、YTHDF2 | inhibition | LPS↑/ METTL3↓/ Smad7 、Smurf1↑/ Runx2↓ | Mouse BMSCs | [84] |
METTL3、YTHDF2 | promotion | piR-36741-PIWIL4 complex↑/ m6A activity of METTL3/ BMP2↑/ Smad1/5/8↑ | Human BMSCs | [85] | |
METTL3↑ | promotion | NOG↓/ Smad1/5↑ | Human MSCs | [86] | |
METTL14↑、IGF2BP1↑ | promotion | SMAD1↑ | Human BMSCs | [87] | |
PI3K/AKT | METTL3↓ | inhibition | Akt phosphorylation↓ | Rat BMSCs | [89] |
ALKBH5↑ | inhibition | PRMT6↓/ p-AKT↓ | Human BMSCs | [90] | |
NF-κB | METTL3↑ | Inhibition | MYD88↑/ NF-κB↑ | Human MSCs | [91] |
ALKBH5 | promotion | MYD88↓/ NF-κB↓ | Human MSCs | [91] | |
MAPK | METTL3↑ | promotion | Pth1r↑/ PKA、ERK↑ | Rat BMSCs | [92] |
AMPK | FTO↑ | promotion | positive feedback loop existed between FTO and p-AMPK/ mild ER stress | Mouse BMSCs | [93] |