Fig. 3

Intra-articular and subchondral bone injection of UCMSCs attenuated subchondral bone loss and enhanced bone formation in OA. (A) Representative sagittal X-Ray images of the knee joints of different groups. (B) Representative 3D reconstructed CT images of tibial subchondral bone of different groups. Scale bar, 250 μm. (C) 3D reconstructed CT images of knees of different groups. Scale bar, 1 mm. (D-G) Quantitative analysis of (D) BV/TV, (E) Tb.Th, (F) Tb.Sp and (G) Tb.N of each group. (H) Representative images of masson staining and IHC staining of different groups. (I) quantitative measurements of masson staining. (J-K) Quantitative analysis of the expression of OCN and CD31. Data presented as means ± SEM by one-way ANOVA with Turkey’s post hoc test. *P < 0.05, **P < 0.01 and ***P < 0.001