Fig. 4

UCMSCs transplant superiorly augmented neovascularization in mouse model for MI. (A, C) Representative images of CD31 and α-SMA staining of heart tissue from mice with MI that received transplantation of saline, UCMSCs or ADMSCs. Scale bar: 200 μm. ( B, D) Quantitative analysis of CD31 and α-SMA density in heart tissue from the different groups on day 28 after surgery and treatment. n = 4 in MI, n = 5 in UCMSCs and ADMSCs, respectively. Scale bar: 200 μm. (E, F) Representative images of CM-DiI-labeled MSCs (Red) in border zones and infarct zones and their quantification on day 7 and day 14 post-MI. n = 3 per group. Scale bar: 200 μm. (G) Representative images of CM-DiI-labeled MSCs and a-SMA double staining on day 14 post-MI. Scale bar: 200 μm. Statistical comparisons were conducted using one-way ANOVA for three groups, and unpaired t test for two-group comparisons. Data are mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. AutoFluo (green) indicates auto fluorescence of cardiomyocytes in heart section. ns indicates non-statistical significance