Fig. 5

ADMSCs had better protective effect against apoptosis in myocardial tissues and in hypoxia H9C2 and HL-1cells. (A, C) Representative images of apoptotic cells on day 1 and day 7 after MI by TUNEL (red) staining. Scale bar: 200 μm. (B) Quantitative analysis of apoptotic cells in heart tissue on day 1 from different groups. n = 4 per group. (D) Quantitative analysis of apoptotic cells in heart tissue on day 7 from different groups. n = 4 per group. (E-G) The protein expressions of Bax and Cleaved caspase-3 and their quantitation in myocardial tissues on day 28 after surgery and treatment by western blotting. (H-J) Apoptosis of H9C2 and HL-1 cells after hypoxia treatment measured by FITC-annexin V/PI double staining analysis using flow cytometry and their qualification from different groups. Statistical comparisons were conducted using one-way ANOVA for three groups, and unpaired t test for two-group comparisons. Data are mean±SD. *p < 0.05, **p < 0.01, ****p < 0.0001. Images of unedited full blots are presented in Supplementary Figure S1