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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cells protect the integrity of the alveolar epithelial barrier through extracellular vesicles by inhibiting MAPK-mediated necroptosis

Fig. 2

MenSCs ameliorate alveolar epithelial barrier dysfunction in ALI. (A) LPS-induced increases in lung permeability were markedly attenuated after MenSC treatment. (B) Relative mRNA expression of tight junction proteins in the lungs of mice administered MenSCs in the presence of LPS was detected via qRT‒PCR. (C) Western blot analysis of the expression levels of the tight junction markers E-cadherin, Occludin, and Claudin-1 in lung tissue. (D) MenSCs ameliorate the LPS induced deterioration of the intercellular junction structure. Lung tissue were immunostained with antibodies against occludin and claudin-1 and visualized under a confocal microscope. (E, F) Quantiication of fluorescence signals. The monolayers immunostained for occludin and claudin-1 in D were analyzed for immunofluorescence intensity by using Image J software

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Stem Cell Research & Therapy

ISSN: 1757-6512