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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cells protect the integrity of the alveolar epithelial barrier through extracellular vesicles by inhibiting MAPK-mediated necroptosis

Fig. 3

MenSCs alleviate LPS-induced alveolar epithelial cell dysfunction in vitro. A non-contact co-culture system was established in vitro between MenSCs and A549 cells. (A) Alveolar epithelial cell permeability was determined in the FD4 supernatant level after MenSC treatment. (B) Relative mRNA expression of tight junction proteins in A549 cells treated with MenSCs in the presence of LPS was detected via qRT‒PCR. (C) Western blot analysis of the expression levels of the tight junction markers E-cadherin, Occludin, and Claudin-1 in A549 cells. (D) The localization of E-cadherin, Occludin, Claudin-1, and ZO-1 in A549 cells was determined by immunofluorescence staining. Scale bar = 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512