Figure 2

Viability and morphology of NC cells embedded in alginate and cultured for four days. Cell viability (a, c) was assessed using LIVE/DEAD staining and morphology (b, d) was observed by means of phase contrast microscopy. Cells retained viability and morphology after FACS (a, b) and after being embedded in alginate for four days (c, d). Cell viability dyes are calcein AM (green) and ethidium homodimer red (red). Scale bar = 200 microns.