Figure 3
Cell-fate tracing of transplanted amniotic fluid stem cells (AFSs) into cleared fat pads. (a) The 2 × 105 AF-derived cells (rapidly adherent within 5 days) and AFSs (slowly adherent, after 5 days of culture) derived from various donors were transplanted into 3-week-old recipient mice and analyzed 8 weeks later as whole mounts. Only the slowly adhering cells selected in the second culture step are able to engraft and can be classified as AFSs. (b) Histologic analysis confirms that the GFP+ AFSs survived and differentiated into adipocytes and connective tissue. Scale bar for whole mounts and tissue sections is 50 μm, (Blue) X-Gal stain or hematoxylin, (Pink) Nuclear fast red, and (Brown) DAB staining.